macspreptm chimerism cd19 microbeads  (Miltenyi Biotec)

Journal: Malaria Journal

Article Title: High purity high yield tandem B and T helper cell isolation for qRT-PCR analysis suitable for basically equipped laboratories

doi: 10.1186/s12936-018-2547-3

Figure Lengend Snippet: List of fluorophore-conjugated antibodies

Article Snippet: Still, other positive B cell isolation kits from whole blood such as the DynabeadsTM CD19 Pan B kit [ ] or the Miltenyi Biotec MACSprep Chimerism CD19 MicroBeads human kit [ ] reside within the same price range with 36–60 USD per isolation and a magnet cost between 1076 USD and 13,200 USD.

Techniques:

Journal: Malaria Journal

Article Title: High purity high yield tandem B and T helper cell isolation for qRT-PCR analysis suitable for basically equipped laboratories

doi: 10.1186/s12936-018-2547-3

Figure Lengend Snippet: Flow cytometry analysis of purity and efficiency of B and Th cell isolation. Shown is a representative analysis of three independent isolations. a Flow cytometry analysis of whole blood after erythrocyte lysis (= before B cell isolation). Upper left: gating of live lymphocytes. Lower left: gating of single cells. Upper right: total T cells (CD3+) versus B cells (CD19+). Lower left: total T cells (CD3+) versus Th cells (CD3+CD4+). b Flow cytometry analysis of isolated CD19+ B cells (= positive fraction after B cell isolation). Upper left: gating of live lymphocytes. Lower left: gating of single cells. Upper middle: total T cells (CD3+) versus B cells (CD19+). Lower middle: total T cells (CD3+) versus Th cells (CD3+CD4+). Upper right: histogram of CD19 positive cells. Lower right: histogram of CD4 positive cells. c Flow cytometry analysis of isolated CD4+ Th cells (= positive fraction after Th cell isolation). Upper left: gating of live lymphocytes. Lower left: gating of single cells. Upper middle: total T cells (CD3+) versus B cells (CD19+). Lower middle: total T cells (CD3+) versus Th cells (CD3+CD4+). Upper right: histogram of CD19 positive cells. Lower right: histogram of CD4 positive cells. d Flow cytometry analysis of the negative fraction after Th cell isolation. Upper left: gating of live lymphocytes. Lower left: gating of single cells. Upper middle: total T cells (CD3+) versus B cells (CD19+). Lower middle: total T cells (CD3+) versus Th cells (CD3+CD4+). Upper right: histogram of CD19 positive cells. Lower right: histogram of CD4 positive cells

Article Snippet: Still, other positive B cell isolation kits from whole blood such as the DynabeadsTM CD19 Pan B kit [ ] or the Miltenyi Biotec MACSprep Chimerism CD19 MicroBeads human kit [ ] reside within the same price range with 36–60 USD per isolation and a magnet cost between 1076 USD and 13,200 USD.

Techniques: Flow Cytometry, Cell Isolation, Lysis, Isolation